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WormLab C. elegans video tracking

JW Janis C. Weeks
WR William M. Roberts
CL Caitlyn Leasure
BS Brian M. Suzuki
KR Kristin J. Robinson
HC Heather Currey
PW Phurpa Wangchuk
RE Ramon M. Eichenberger
AS Aleen D. Saxton
TB Thomas D. Bird
BK Brian C. Kraemer
AL Alex Loukas
JH John M. Hawdon
CC Conor R. Caffrey
NL Nicole F. Liachko
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Stage matched N2 worms were grown at 20 °C to day 1 of adulthood. Approximately 50 day 1 adults were transferred onto NGM plates with drugs at the indicated concentrations for 18 hours. Using the video capture and analysis features of the software WormLab (Version 4.1, MBF Bioscience, Williston, VT, USA), 60 s video recordings with a resolution of 2592 × 1944 pixels were taken at 14 frames/s using a Basler acA2500 digital camera. Worms with tracking times greater than 30 s were used in the final analysis. Straight-line speed was calculated from the positional change starting at the beginning of track to end of track (µm) and then divided by track recording duration (s). Each drug concentration was tested in triplicate and results averaged.

Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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