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Mice were anaesthetized under a 2.5% oxygen flow containing 1.5–2% isoflurane. Nerve conduction studies were performed using sub-dermal needle electrodes (Technomed Europe, Maastricht, Netherlands) and a Natus UltraPro S100 (Natus Medical Incorporated, San Carlos, CA, USA). Compound muscle action potentials (CMAPs), were determined by measuring the response at the gastrocnemius muscle after stimulation occurred at the level of the sciatic notch.
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