Colony formation assay

NZ Nana Zheng
LW Lixia Wang
YH Yingying Hou
XZ Xiuxia Zhou
YH Youhua He
ZW Zhiwei Wang
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PC3 and DU145 cells were seeded in 6 cm2 dishes at 1 × 103 cells/well with 3 μM and 5 μM concentrations of rottlerin. After two weeks, 4 % paraformaldehyde was added to fix the cells and crystal violet was used to stain the colonies.

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