Genomic gene expression profiling

NG N. M. Grindler
LV L. Vanderlinden
RK R. Karthikraj
KK K. Kannan
ST S. Teal
AP A. J. Polotsky
TP T. L. Powell
IY I. V. Yang
TJ T. Jansson
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RNA was isolated using the Qiagen AllPrep DNA/RNA Mini kit. RNA integrity was established as RNA Integrity Number (RIN) >8. Total RNA was reverse-transcribed into single-stranded cDNA and then cRNA using random primers in the One-Color Microarray-Based Low Input Quick Amp Labeling (Agilent). Gene expression profiling was performed using microarray (Agilent Sureprint G3 8 × 60 K array).

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