RNA was isolated using the Qiagen AllPrep DNA/RNA Mini kit. RNA integrity was established as RNA Integrity Number (RIN) >8. Total RNA was reverse-transcribed into single-stranded cDNA and then cRNA using random primers in the One-Color Microarray-Based Low Input Quick Amp Labeling (Agilent). Gene expression profiling was performed using microarray (Agilent Sureprint G3 8 × 60 K array).
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