Oral glucose tolerance test (OGTT)

After keeping on 18 h fasting, mice were placed into four groups (for both compounds total eight groups). The sample size in each group comprised of five mice. Group I and II were non-diabetic and diabetic control, injected with saline (10 mL/Kg) and alloxan (300 mg/Kg) respectively. Group III was treated with the test compound (DPAA, 10 mg/Kg/DPC1, 5 mg/Kg). Group IV was positive control and injected with metformin (500 mg/Kg). All groups were pre-treated and after 30 min. D-glucose load of 2 g/Kg was given orally. Blood glucose levels were measured at 0, 30, 60, 90, and 120 min., using Easy Gluco auto-coding glucometer (Marguet et al., 2000).