Chi-square tests were used to compare the distribution of stages of eggs laid by wild-type and mutant animals. Unpaired t-tests were used to compare cyp-36A1 mRNA expression between strains, survival on tunicamycin between strains, survival on t-BOOH between strains, and PT24B8.5::gfp reporter fluorescence intensity between strains. Log-rank (Mantel-Cox) tests were used to compare survival of different strains on Pseudomonas aeruginosa. In cases of multiple comparisons, a Holm-Bonferroni correction was applied. Statistical tests were performed using GraphPad Prism software (Graphpad Prism, RRID:SCR_002798) version 7.0a. Biological replicates were performed using separate populations of animals.
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