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Parasite Quantification

SB Sarah A. Budischak
CH Christina B. Hansen
QC Quentin Caudron
RG Romain Garnier
TK Tyler R. Kartzinel
IP István Pelczer
CC Clayton E. Cressler
AL Anieke van Leeuwen
AG Andrea L. Graham
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Caeca were cut open longitudinally and examined under a dissecting microscope by an observer blind to infection status. Worms were isolated by scraping the gut mucosa into a series of clean petri dishes of water. After enumeration, worms were stored in 70% ethanol for subsequent length measurements. Each worm was photographed under a dissecting scope (2–4× power) and ImageJ (version 1.49, NIH, USA) was used to measure total length.

Copyright and License information:  ©2018 Budischak, Hansen, Caudron, Garnier, Kartzinel, Pelczer, Cressler, van Leeuwen and Graham.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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