Xenograft mouse model of ovarian carcinoma:

Animal studies were carried out according to the protocol approved by the Institutional Animal Care and Use Committee (IACUC) at Massachusetts General Hospital (MGH). A subcutaneous xenograft mouse model of human ovarian cancer (OVCAR-5) was established by implanting 1.5 × 10⁶ OVCAR-5 cells (suspended in a medium-Matrigel mixture; 100 μL) into the right hind flank of 6-week old female Swiss nude mice. Tumors were allowed to grow for two weeks, at which point treatment was initiated. Tumor size was record using calipers and tumor volume estimated using the formulation V = (L × W × H) π/6, where V is tumor volume, W is tumor width, L is tumor length, and H is tumor height.