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5-Azacytidine (5-AzaC) demethylation treatment
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For demethylation studies, HepG2 and SMMC-7721 cells were treated with the indicated concentrations of 5-AzaC (Sigma-Aldrich, St. Louis., MO, USA) for 36 h. The cells were then harvested for qPCR and western blot analysis.
Copyright and License information: ©2016 Wu et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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