Materials

Soya phosphatidyl choline (LIPOID S 100; SPC) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Ethyl polyenoate (EP) was obtained from Shandong Yuwang Pharmaceutical Co., Ltd. (Yucheng, China). Cab was supplied by Guangzhou Zhiyuan Pharmacy Co., Ltd. (Guangzhou, China). Sodium cholesterol sulfate (SCS) was purchased from Shanghai Haoran Biotechnology Co., Ltd. (Shanghai, China). BioTL was purchased from Genewiz, Inc. (Beijing, China). Tris (hydroxymethyl) methyl aminomethane (Tris) was obtained from Jian Yang Biotechnology Co., Ltd. (Guangzhou, China). The nucleotide sequence of TLS1c is 5′-ACAGGAGTGATGGTTGTTATCTGGCCTCAGAGGTTCTCGGGTGTGGTCACTCCTG-3′. TLS1c was purchased from Genewiz, Inc.; biotin was conjugated with TLS1c at the 3′ end. Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) provided 6-carboxy fluorescein (6-FAM). Harvey Biotech Co., Ltd. (Beijing, China) provided 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). HepG2, macrophages and MEAR cells were provided by Shanghai Fuxiang Biotechnology Co., Ltd. (Shanghai, China). Caco-2 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). The TDL-40B Ultrafiltration centrifuge was supplied by Shanghai Anting Science Technology Instrument Factory (Shanghai, China). All other chemical and buffer components were of analytical grade.