Serum and urinary CD80 and CTLA-4 were detected when patients were in relapse or complete/partial remission, and 24 h urinary protein, uPCR and serum albumin were measured in the same day. CD80 and CTLA-4 levels were measured using a commercially available ELISA kit (Bender MedSystems, eBioscience, Vienna, Austria), and results were adjusted for urinary creatinine excretion. Urinary creatinine level and protein and serum albumin levels were measured using an autoanalyzer.
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