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Total soluble solids (TSS) and titratable acidity (TA)
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The TSS content in the extracted strawberry juice was measured by a refractometer (ATAGO Co., Tokyo); the results were expressed as °Brix. TA was measured for each 2 mL strawberry juice sample; 0.1 mol L−1 NaOH was used to titrate the juice with a semi-automatic titrator (Multi-Dosimat E-415 titrator, Metrohm AG, Switzerland) to a phenolphthalein endpoint of pH 8.1. TA was expressed as the percentage of citric acid equivalents and three independent replicates were performed.
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