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Caspase-3 activity assay
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The left cerebral hemispheres of brain tissues were isolated for caspase-3 activity assay. The tissues were homogenized, and then centrifuged at 20,000 × g for 15 min at 4 °C. Activity of caspase-3 was measured using a caspase-3 activity test kit (Abcam). According to the manufacturer’s instructions, 100 μg of total proteins in 100 μl total volumes was mixed with 100 μl of equilibrated test Caspase-Glo reagent and incubated for 1 h at 37 °C. The caspase-3 enzyme activity was measured using a luminometer reader (Beckman Co., Brea, CA, USA).
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