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Nanoparticle tracking analysis

BY Bo Yuan
CR Christos Ritzoulis
JC Jianshe Chen
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The sizes and the size distributions of all macromolecular populations were quantified with a Malvern NanoSight NS300 nanoparticle-tracking analyzer (Malvern instruments, Worcestershire, UK). Samples were passed through a 0.45 μm syringe filter before their insertion into the device’s measuring chamber. The conditions were: continuous phase viscosity 1 mPa s and temperature 25 °C. After successive dilutions and trial and error measurements using the appropriate buffers, results were recorded for 1:1 v/v okra polysaccharide (OP)–AS solutions, at a 0.05% final OP content. Measurements were taken for 60 s per run. Measurements were performed in triplicate.

Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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