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A549 cells were grown on Poly-L-lysin coated coverslips in 6-well tissue culture plates. Cells were incubated on ice for 30 minutes to depolymerize microtubules. Next, cells were treated with two different concentrations of the compound or DMSO and placed at 37 °C for 10 minutes. Cells were then fixed with ice-cold methanol and stained for immunofluorescence using alpha-tubulin antibodies as described above.
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