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Analysis by In Vivo Imaging System (IVIS)

SB Sarp Bamyaci
RN Roland Nordfelth
ÅF Åke Forsberg
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Emitted light from infecting bacteria was monitored and analysed by using a Spectrum In Vivo Imaging System Instrument (Caliper Life Sciences/PerkinElmer). Every second day, mice were anaesthetized with a 25% mixture of Isofluorane (IsoFlo vet: Orion Pharma) in oxygen and thereafter placed in the IVIS under continued isofluorane anaesthesia. Images generated were analysed with the Living Image software, version 4.5.4 (Caliper Life Sciences/PerkinElmer). Bioluminescent signals emitted by the bacteria were determined from defined regions of interest (ROIs) with identical sizes from organs dissected out from the mice and expressed as total Flux (photons per second).

Copyright and License information:  ©2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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