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SO Satu Oltedal
IS Ivar Skaland
JM Jodi Maple-Grødem
KT Kjersti Tjensvoll
EJ Emiel A. M. Janssen
BG Bjørnar Gilje
RS Rune Smaaland
RH Reino Heikkilä
ON Oddmund Nordgård
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Expressed sequence tag (EST) databases at the Cancer Gene Anatomy Project (CGAP; US National Cancer Institute) were searched for potential colorectal cancer biomarkers using the cDNA digital gene expression displayer (DGED) tool in June 2004. Libraries from normal colon and colon cancers (including cell lines) were compared to all other available libraries.

Multiple sequence alignments were analyzed using MUSCLE 3.51 software. The alignment was colored by JalView version 2.7. Homology searches were performed with the blastn, blastp, tblastn, Phi-blast, PSI-blast, Delta-blast and HMMER3 software programs in the Genbank nr and Swissprot databases. The subcellular localization of PHGR1 was predicted by PSORT II. No clear predictions were possible from the primary sequence of PHGR1 (data not shown).

Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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