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Full length cDNA sequences of PmDAMs and PmCBFs were amplified through PCR using specific primers (Supplementary Table S3). These sequences were cloned into pGBKT7 bait vector and pGADT7 prey vector at the EcoRI and BamHI sites, respectively, using an In-Fusion HD Cloning Kit System (Clonetech, USA). The Y2H assays were performed by former methods42. Each interaction analysis was performed three times.
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