The Agilent 1200 series high performance liquid chromatography system (HPLC) was used for molecular mass determination employing the TSKgel G2000SWXL 6 x 250 mm column (Tosoh Bioscience). Flow rate was adjusted to 0.5 ml/ml. UV 215 was used for sample detection. Reference samples of molecular mass standards were obtained from BioRad and Sigma.
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