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The hanging wire test was used to monitor muscle function over time41. Briefly, a 55-cm-wide, 2-mm-thick metal wire was fixed at two vertical stands. Mice were allowed to grasp the middle of the wire with their fore limbs and turn upside-down along the wire axis. A timer was started when the mouse was grasping the wire with its four paws. Each holding time was recorded. The average holding time was obtained from three trials. The holding impulse [mass (gram) × hang time (sec)] was used as an outcome measure.
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