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Creation of pancreatic cancer xenograft animals

MG Mei Gao
ML Miranda Lin
RM Richard A. Moffitt
MS Marcela A. Salazar
JP Jinha Park
JV Jeffrey Vacirca
CH Chuan Huang
KS Kenneth R. Shroyer
MC Minsig Choi
GG Georgios V. Georgakis
AS Aaron R. Sasson
MT Mark A. Talamini
JK Joseph Kim
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Stony Brook University Institutional Animal Care and Use Committee approved the animal studies, which utilized 6–12-week old NSG mice (The Jackson Laboratory). To create PDTXs, we utilized a standard operating procedure to implant tissues into mice within 30 min of surgical excision.23 In brief, PDACs were removed en bloc in the operating room, taken to pathology, and then distributed by a surgical pathologist to provide portions for PDO and PDTX development. For PDTX, tissues (20–30 mm3) were implanted subcutaneously into both left and right flanks of mice designating passage 0. About 2–4 months later with positive tumor growth, tumor tissues were harvested and split into three mice denoting passage 1. Thereafter, growing tumors were further expanded into mice designating passage 2 for drug treatment studies.

Copyright and License information: Cancer Research UK ©2018
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