Antibodies

Goat anti‐TIAR (C‐18) (sc‐1749), rabbit anti‐Sam68 (sc‐333), mouse anti‐HuR (sc‐5261), mouse anti‐RNase H1 (sc‐136343), mouse anti‐CDK1 (Cdc2, sc‐137034), mouse anti‐CDK2 (sc‐163), mouse anti‐PCNA (sc‐56), rabbit anti‐GFP (sc‐8334), mouse anti‐Cdc25B (sc‐1619), rabbit anti‐Rad51 (sc‐8349), goat anti‐TIA1 (sc‐1671), and goat anti‐eIF3B (eIF3eta) (sc‐16377) are from Santa Cruz; rabbit anti‐CENP‐A (ab13939), mouse anti‐actin (sc‐47778), rabbit anti‐FANCD2 (ab2187), rabbit anti‐Lamin‐B1 (ab16048), mouse anti‐pS2‐RPOL2A (H5) (ab24758), rabbit anti‐γ‐tubulin (ab11317), rabbit anti‐H3 (ab1791) and rabbit anti‐p‐H3 (phospho‐Ser10) (ab1136535), and rat anti‐BrdU (ab6326) are from Abcam; rabbit anti‐RPA1 (2267), rabbit anti‐p‐Lamin A/C (phospho‐Ser22) (13448), rat anti‐RPA2 (2208), rabbit anti‐p(T161)‐Cdk1 (9114), rabbit‐p(Y15)‐Cdk1 (9111), and mouse anti‐Cyclin B (4135) are from Cell Signaling; mouse anti‐γH2AX (JBW301) and mouse anti‐ssDNA antibody (MAB3034) are from Millipore; rabbit anti‐53BP1 (100‐304A2) is from Novus Biologicals; mouse anti‐α‐tubulin (T9026) is from Sigma; mouse anti‐BRCA1 (OP92 Ab1) is from Calbiochem; mouse anti‐Lamin A/C (477BD7) is from New England Biolabs; and mouse anti‐BrdU ({"type":"entrez-nucleotide","attrs":{"text":"BD347580","term_id":"92239934"}}BD347580) is from BD Biosciences. Mouse monoclonal anti‐Sm (Y12) antibody 70 was kindly provided by Iain Mattaj (EMBL Heidelberg, Germany), rabbit anti‐Coilin was kindly provided by Angus I. Lamond (University of Dundee, UK), and rabbit polyclonal anti‐PRP19 antibody has been described previously 71.