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Tumor Volume Determination from Digital Caliper Measurements

AB Andrew Brannen
ME Matthew Eggert
MN Matthias Nahrendorf
RA Robert Arnold
PP Peter Panizzi
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Tumor growth was assessed three times per week using digital caliper measurements of tumor x/y dimensions. Prior to imaging sessions, mice received anesthesia using 1–3% isoflurane gas with medical-grade oxygen while three independent reviewers measured tumor dimensions to determine a mean volume as a basis for correlation. Volumes were calculated using a standard formula derived from the volume of an ellipsoid,

Humane endpoints were defined as tumors that approached or exceeded 1500 mm3. Otherwise, tumors were monitored for 9 weeks before mice were euthanized. Tumors were resected and then measured relative to three axial dimensions of diameter, and volumes were calculated using

Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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