Modeling

Structure models of the N-terminal domains were generated by Phyre2 (Kelley et al., 2015). A structural model of an intact TnpA^ISC1926 dimer was generated from the Phyre2 model of the TnpA^ISC1926 NTD (residues 12 – 61, Figure 2—figure supplement 2C) linked to residue 65 of the CTD by the native residues Arg-Glu-Glu using Coot. The NTD was docked onto a DNA model (3DNA, [Lu and Olson, 2003]) of the IS1535 LE sequence with the aid of the closely related RacA-DNA complex (Figure 2—figure supplement 2C) and DMS protection data (Figure 8—figure supplement 1) using PyMOL and Coot.