Cell culture and transfection

Human hepatoma cell lines Huh-7 and HepG2 were obtained from the ATCC (Manassas, VA, USA). The cell lines were authenticated by short tandem repeat DNA testing at Beijing Microread Gene Tech., Co., Ltd. in 2015. Huh-7 and HepG2 were cultured in Dulbecco's Modified Eagle's medium. Transfections were performed using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). Cells were transfected with 50 nM siRNA or 2ug expression plasmid. Each treatment was performed at least three times.