Confocal Microscopy of MYB107-GFP

Both pGWB405:MYB107 (MYB107:GFP) and the empty vector pCAMBIA1302 (free GFP) were transformed into Agrobacterium tumefaciens strain GV3101. Six-week-old Nicotiana tabacum plants were infiltrated with an A. tumefaciens strain harboring each of the constructs. Fluorescence images were captured via a Leica TCS SP5 Laser Scan Confocal Microscope with excitation at 488 nm; an emission signal between 493 and 560 nm was collected for GFP fluorescence, and excitation at 496 nm and an emission signal of 630 to 720 nm were collected for chlorophyll autofluorescence.