Isolation, screening and identification of novel MDR isolates

MDR bacterial isolates were screened from clinical samples collected from Assam Medical College Hospital, Dibrugarh by observing their resistance patterns against at least four antibioticsout of the following: Metropenem (10mcg), Polymixin B (10mcg), Metronidazole (5mcg), Novobiocin (30mcg), Cefixime (5mcg), Ciprofloxacin (5mcg), Tetracycline (30mcg), Gentamycin (30mcg), Amikacin (30mcg), Rifampicin (5mcg), Erythromycin (15mcg), Ampicillin (10mcg), and Imipenem (10mcg). The isolate showing resistanceagainst maximum number of antibiotics was then identified by 16S rDNA sequencing technique using 704F forward primer (5’-GTAGCGGTGAAATGCGTAGA-3’) and 907R reverse primer (5’-CCGTCAATTCMTTTGAGTTT-3’).