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Culture and differentiation of hiPSC-RPE
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HiPSC (IMR90-57) were cultured on mouse embryonic fibroblasts (SiDan-Sai Biotechnology Co., Ltd, Shanghai, China) in six-well tissue culture plates as detailed elsewhere.3 The hiPSCs were then differentiated into RPE cells according to the SFEB/CS method using low-molecular-weight compounds CKI-7 (5 μM) and SB-431542 (5 μM).36
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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