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The chow diet was the Teklad LM-485 mouse/rat diet (7012) and the high fat diet was D12451 from Research Diets Inc. The high fat diet provided 45% of the calories from fat, while the chow diet contained 5% of the calories as fat. The mice were given free access to food throughout the study. The food was weighed at weekly intervals and the kilocalorie consumption was calculated based on grams of food eaten, the calorie density of the food, and the weight of the mice.
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