Quantification of thromboxane A2 and prostacyclin I2

AB Alexandra Boivin
MB Mélanie Burban
RC Raphaël Clere-Jehl
PB Pierrick Le Borgne
HM Hamid Merdji
CA Cyril Auger
VS Valérie Schini-Kerth
FM Ferhat Meziani
JH Julie Helms
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Thromboxane B2 (TXB2), derived from rapid non-enzymatic hydrolysis of TXA2, and prostacyclin I2, which is rapidly non-enzymatically hydrated to a stable product, 6-keto PGF1α, were measured in plasma using the respective immunoassay kit (ELISA) according to manufacturer’s instructions (Cayman Chemical Company, Ann Arbor, MI, USA).

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