Thromboxane B2 (TXB2), derived from rapid non-enzymatic hydrolysis of TXA2, and prostacyclin I2, which is rapidly non-enzymatically hydrated to a stable product, 6-keto PGF1α, were measured in plasma using the respective immunoassay kit (ELISA) according to manufacturer’s instructions (Cayman Chemical Company, Ann Arbor, MI, USA).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.