At the end of the experiment, all rats were anesthetized with pentobarbital sodium (30~40 mg/kg, i.p.) and a catheter was cannulated into the carotid artery to take blood samples (~8 ml each) for the measurement of plasma renin activity and plasma angiotensin I, II and aldosterone concentrations. Plasma renin activity and plasma angiotensin I, II and aldosterone concentrations were measured using the RIA2 method (SRL, Tokyo, Japan).
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