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Total EAAT1 and EAAT2 RNA was isolated from cultured MSCs in astrocytic or control mediums using QIAzol reagent (Qiagen, CA). 0.5μg of RNA was used to synthesize cDNA by Thermoscript (Invitrogen) with oligo dT primers, followed by SYBR green qPCR method (primers and further details in Supplementary Information (SI)).
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