Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
In vitro immunofluorescence quantification
Ask a
question
Favorite
Quantification of neurites was achieved by staining PFA‐fixed cortical and hippocampal neurons with a mouse MAP2 antibody (1:1000, Millipore). Sample digital images of MAP2 stained neurons of a fixed area were obtained from the same location in the 24‐well plate of control or experimental cultures blindly and the amount of immunofluorescence was quantified utilizing ImageJ analysis software.18 Data were expressed as the amount of MAP2 stain per unit area. A single N value represents average stain quantified per area per a single culture well.
Copyright and License information: ©2017 The Authors. published by Wiley Periodicals, Inc on behalf of American Neurological Association.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
