Cell Viability Assays

Endpoint viability assays were performed using the CellTiter-Glo (Promega) assay according to the manufacturer's protocol. Briefly, cells were seeded at 600 to 800 cells per in 384-well plates or 2,000 cells in 96-well plates and allowed to establish overnight before adding treatments. Cells were treated for 6 to 7 days with compounds, over a range of concentrations, then the assay was terminated and viability assessed using luminescence detection on a Victor X4 plate reader (Perkin Elmer). Luminescence was normalized to DMSO control, and IC₅₀ values were calculated using a sigmoidal dose–response curve fit analysis (Prism software, GraphPad).