Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Antibodies.

SH Sam Horng
AT Anthony Therattil
SM Sarah Moyon
AG Alexandra Gordon
KK Karla Kim
AA Azeb Tadesse Argaw
YH Yuko Hara
JM John N. Mariani
SS Setsu Sawai
PF Per Flodby
EC Edward D. Crandall
ZB Zea Borok
MS Michael V. Sofroniew
CC Candice Chapouly
GJ Gareth R. John
request Request a Protocol
ask Ask a question
Favorite

Catalog numbers of all antibodies from their respective manufacturers are shown in parentheses. Anti-CLDN1 (374900, mouse), anti-CLDN4 (329400, mouse), anti-CLDN5 (352500, mouse), anti-cingulin (PA31654, rabbit), anti-GFAP (130300, rat), and anti-mouse IgG (A11029, goat) were from Invitrogen. Anti–ZO-1 (617300, rabbit) was from Life Technologies, and anti–ZO-2 (rabbit) was from Zymed. Invitrogen, Life Technologies, and Zymed are all divisions of Thermo Fisher Scientific. Anti–JAM-A (sc-25629, rabbit) and anti–β-actin (sc-47778, mouse) were from Santa Cruz Biotechnology. Anti–JAM-A (564908, rat) was from BD Biosciences. Anti-fibrinogen (A0080, rabbit) was from Dako. Anti-CD3 (16-0037-85), anti-CD4 (14-9766-82), anti-CD11b (14-0112-82), and anti-CD45 (550539, all rat) were from eBioscience. Anti-NeuN (MAB377, mouse) and anti–myelin basic protein (anti-MBP) (MAB386, rat) were from Millipore. Anti-laminin (L9393) and anti-afadin (A0349, both rabbit) were from Sigma-Aldrich. Anti-MUPP1 (611558, mouse) was from BD Transduction. Anti-CASK (AM20099PU-N, mouse) was from Acris.

Copyright and License information:  ©2017, American Society for Clinical Investigation

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A