4.5. Quantification of elastase and pyoverdine

Bacterial strains were grown in ABT minimal medium [50] supplemented with 5 g l^–1 glucose and 2 g l^–1 casamino acids. The filtered supernatants of overnight culture were used to quantify the productions of elastase and pyoverdine in vitro. The quantification of elastase production was performed in a 96-well plate using EnzCheck Elastase Assay Kit (Thermo Fisher Scientific) as instructed by the manual. Elastase activity was measured by Infinite M200 PRO system (Tecan) and the results were shown as emission at 530 nm. For the quantification of pyoverdine, filtered supernatant was measured for fluorescence signal emitted at 460 nm upon excitation at 398 nm using Infinite M200 PRO system (Tecan). Both experiments were performed in at least triplicates and the results are shown as mean ± standard deviation in the figures.