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Isolation of bone marrow neutrophils (BMNs) and blood neutrophils (BNs)

SF Svetlana A. Fayngerts
ZW Zhaojun Wang
AZ Ali Zamani
HS Honghong Sun
AB Amanda E. Boggs
TP Thomas P. Porturas
WX Weidong Xie
ML Mei Lin
TC Terry Cathopoulis
JG Jason R. Goldsmith
AV Anastassios Vourekas
YC Youhai H. Chen
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Murine blood was drawn from retro-orbital plexus of the eyes, and bone marrow was collected from femur and tibia bones. BNs and BMNs were isolated using Histopaque-1119 and Histopaque-1077 (Sigma-Aldrich) according to the manufacturer’s instructions. The purity of BNs and BMNs populations was greater than 90% as determined by flow cytometry after staining with anti-Ly6G-APC (eBioscience), and the viability was greater than 99% as determined by trypan blue staining.

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