Sequencing of the cosmid clone containing the putative surfactin biosynthesis genes

Positive cosmid clones, pJJ815 and pJJ121, were selected by colony hybridization and Southern hybridization. Restriction enzyme maps of the cosmid clones were constructed by digestion with EcoRI and SmaI, and then EcoRI digested fragments of the cosmid clones were subcloned into the high-copy vector pTZ18R. The sequences of both strands of the vector constructs were confirmed by sequencing on an ABI 3730XL capillary DNA sequencer (Solgent), and the nucleotide sequencing results were analyzed by using NCBI BLAST and CLUSTALW.