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Live/Dead Cell staining

GR Girdhari Rijal
WL Weimin Li
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The survival of the cells grown on the 3D scaffolds was assessed using the Live/Dead Cell Staining Kit. The cell-carrying scaffolds at different time points or under different treatment conditions were collected and manually sectioned at a thickness of 250 to 500 μm. The sections were washed twice with 1× PBS (37°C), incubated in the staining solution (2 μM calcein-AM and 4 μM EthD-III in 1× PBS) at RT for 45 min, and imaged using fluorescence microscopy. Calcein-AM stains live cells green (under EGFP filter), whereas EthD-III stains dead cells red (under Texas Red filter).

Copyright and License information:  ©2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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