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Blue native polyacrylamide electrophoresis (BN-PAGE)
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The method was adapted from Hornikova et al [56]. Briefly, purified nanostructures VarC were mixed with native PAGE sample buffer (ThermoFisher Scientific) and separated in a native 3–12% gradient gel. The separated complexes were visualized using Gelcode staining.
Copyright and License information: ©2017 Fraiberk et al
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