SUnSET Protein Synthesis Assay

SUnSET was performed as described (Ma et al., 2014). Hippocampal slices were incubated with puromycin (1 g/ml) for 60 min throughout the drug-treatment experiments. For the in vitro experiments, slices were treated with 100 pM GLP-1 (9-36). At the end of drug treatment slices were harvested and frozen on dry ice; protein lysates were prepared for Western blotting. Puromycin-labeled proteins were identified using the mouse monoclonal antibody 12D10 (1:10,000; Millipore). Protein synthesis levels were determined by taking total lane density in the molecular weight range of 10– 250 kDa.