Selection of NPs.

A smart chemical library containing 199 natural products (NPs) with potential antifungal activity was constructed. Among these compounds, 53% were previously isolated from crude plant extracts that presented antifungal activity. The bioguided process of isolation of these compounds was performed by bioautography using wild-type and genetically modified strains of C. albicans (12). In parallel, NPs with (i) structures closely related to those of NPs possessing antifungal activity from published sources and (ii) unknown antifungal activity were selected from commercial catalogues and acquired (the compounds are listed in Table S1 in the supplemental material). The identity and the purity of the commercial compounds obtained were systematically determined by nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) analysis.