2.8. Aerobic Plate Counts

All samples were subjected to microbiological analysis. One gram of shrimp muscles were added into peptone water (0.1%), then the samples were homogenized for 1 min, and the samples were then serially diluted in 9 mL peptone water (0.1%). The aerobic plate counts were enumerated by spread-plating of 1 mL of sample solution on sterile Petri-plates containing plate count agar (Difco Laboratories, Detroit, MI, USA). Plates were incubated for 24 h at 35 ± 1 °C [22].