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GUS histochemical analysis

ZJ Zhimin Jiang
GX Gang Xu
YJ Yanjun Jing
WT Weijiang Tang
RL Rongcheng Lin
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The RVE1p:GUS homozygous transgenic seeds were subjected to various light conditions as indicated in the text. The embryo and endosperm were dissected under a stereomicroscope (Olympus) in green light. The samples were incubated in 0.1 M sodium phosphate buffer containing 50 mM K3Fe(CN)6, 50 mM K4Fe(CN)6 and 1 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronide at 37 °C for 3–6 h. GUS staining was examined under a stereomicroscope and images were captured by a digital camera (Olympus).

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