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For immunoprecipitation of BAG3 protein the anti-BAG3 mAb AC-2 was coupled to Dynabeads (Invitrogen) following the manufacturer's instructions. Briefly, 500 × g of cell extracts were immunoprecipitated at 4°C overnight and then analysed by Western blot using a rabbit anti-BAG3 polyclonal primary antibody, anti-BRAF antibody, anti-HSC70 antibody, and anti-GAPDH antibody.
Copyright and License information: ©2017 Guerriero et al.
This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
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