Image management and statistical analysis.

Immunoblot and confocal micrographs were prepared in Photoshop CS5, and figures were constructed in Illustrator CS5.1 (Adobe Systems, San Jose, CA). All images were minimally processed, adjustments were applied equally across each image, and no adjustment was made that resulted in data loss. For immunoblot graphing and statistics, films were imaged and quantitated using ImageJ, and the t-test function in GraphPad5 (Graph Pad Software, La Jolla, CA) was used. For quantitation of signals in conditioned media, signals were normalized to the protein concentration from the correlated lysate. For quantitation of signals from lysate, signals were normalized to the signal from GAPDH or β-actin control staining.