Mouse model of skin wound infected with MRSA

In the experiment, adult male ICR mice (obtained from Vital River Laboratory Animal Technology Co. Ltd., Beijing), aged 6–8 weeks, were utilized. The protocols used in animal experiments were approved by the Laboratory Animal Centre of Peking University, China. The methods were carried out in accordance with the relevant guidelines, including any relevant details. 3% pentobarbital sodium was injected into intraperitoneal to anesthetize mice before the dorsal surfaces being shaved. A skin site with a 1 cm × 1cm wound was made by sterile syringe needle with the diameter of 0.4 mm. The depth of puncture is about 1 mm marked on the needle by marking pen. After wounded, 50 μL of MRSA suspensions (~10⁸ CFU/mL) was inoculated over the wound site. To verify mice skin infection model, bacterial culture and biochemical identification were performed. Mice were randomly divided into four groups, each group had six mice.