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The Mtwo R25/0.05 instruments were used in the crown-down technique. The instruments were activated by the torque control endodontic motor (X-Smart, Dentsply Maillefer) at 280 rpm and 120 g/cm−1 torque according to the manufacturer's instructions. The files were used with a brushing motion against the lateral walls. The final apical preparation was achieved using an Mtwo (50/0.04) file (VDW) at 200 rpm and 200 g/cm−1 torque.

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