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Pri-miR-375 was amplified by PCR using the sense primer 5′-GGATCCGTGTCAGCCGCAGATGCG TTCAG-3′ and anti-sense primer 5′-CTCGAGCTAGA ATCCGGGTTTCCACCTCCAG-3′. The PCR products were cloned into BamH I/EcoR I sites of a lentiviral vector pCDH-CMV-MCS-EF1-GFP-T2A-Puro (pCDH-puro, SBI, CN).
Copyright and License information: ©2017 Wei et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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