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In vitro invasiveness of the ovarian cancer cells was assessed by quantifying the number of cells that invaded the Matrigel. Polycarbonate Transwell filters was coated with ~100μg/100μL of diluted Matrigel solution. Then, the cells (2 × 104) were added to the upper chamber with either DMSO or 100μM SsaI and 10% FBS medium was added as a chemoattractant in the lower chamber followed by incubation at 37°C for 24 h. The cells that travelled through the filter were stained and counted from 7 to 10 random fields of view at 400 × magnification.
Copyright and License information: ©2017 Wen et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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